摘要
目的研究新型纳米根管充填材料纳米羟磷灰石/聚酰胺66(nHA_PA66)对成骨细胞的生物学作用。方法以含nHA_PA66的细胞培养基(DMEM)浸提液作用于实验组细胞,DMEM培养基作用于对照组细胞,采用MTT比色法检测nHA_PA66对成骨细胞生长的影响;酶联法检测细胞ALP活性的变化;QRT_PCR测量细胞骨钙素基因表达的变化。结果实验组细胞的相对增殖率在98%～106%之间,且无量效关系,实验组与对照组间无显著性差异(P>0.05);实验组和对照组细胞的ALP活性及骨钙素基因表达相似,皆无显著性差异(P>0.05)。结论nHA_PA66对成骨细胞的生长和功能无不良影响,具有骨细胞相容性。
Objective To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.Methods MTT assay was used to determine the growth of osteoblast, enzymatic measure was used to determine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in osteoblasts treated by DMEM eluate of nHA-PA66.Results Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98%～106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P>0.05).Conclusion nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compatibility is proved.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第2期142-144,共3页
West China Journal of Stomatology
基金
四川省科委重点资助项目(02SG022_001)
