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植物中超氧阴离子自由基测定方法的改进 被引量：157

Improvement of Measurement Method for Superoxide Anion Radical in Plant
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摘要 通过对植物超氧阴离子自由基测定反应中动力学曲线的分析, 确定了最佳的反应介质、反应参数和羟胺浓度, 以三氯甲烷代替乙醚作为植物色素萃取试剂, 克服了植物超氧阴离子测定中存在的诸多问题, 提高了测定结果的准确性、重复性和可比性。 This paper analysed kinetics curve of superoxide anion radical reacting with hydroxylamine.Optimum reaction medium,reaction parameters and hydroxylamine concentration were confirmed.The result indicated that chemical reagent chloroform instead of ethyl ether as extraction reagent of plant pigment,overcomed some problems of measurement method for superoxide anion radical in plant,and enhanced the accuracy,reproducibility and comparability.
作者 李忠光 龚明
机构地区 云南师范大学生命科学学院
出处 《云南植物研究》 CAS CSCD 北大核心 2005年第2期211-216,共6页 Acta Botanica Yunnanica
基金 国家自然科学基金 (30460016) 云南省自然科学基金 (2004C0031M) 国际合作计划(2002GH06) 教育部高校优秀青年教师教学科研奖励计划(2001年度)
关键词 植物 超氧阴离子自由基 测定方法 Plant Superoxide anion radical Measurement method
分类号 Q94-3 [生物学—植物学]
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参考文献8

  • 1王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,26(6):55-57. 被引量：1520
  • 2Elstner EF,Heupel A,1976.Inhibition of nitrite formation from hydroxylammoniumch-loride:a simple assay for superoxide dismutase[J].Anal Biochem,70:616-620.
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  • 4李忠光,杜朝昆,龚明.在单一提取系统中同时测定植物ASA/DHA和GSH/GSSG[J].云南师范大学学报（自然科学版）,2003,23(3):67-70. 被引量：34
  • 5李忠光,龚明.抗氧化系统在H_2O_2诱导的玉米幼苗耐热性形成中的作用[J].植物生理学通讯,2003,39(6):575-579. 被引量：10
  • 6Peters RR,Krepsky PB,Siqueira-Junior JM,et al,2003.Nitric oxide and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata[J].Life Sciences,73:2185-2197.
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二级参考文献34

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共引文献1557

同被引文献2411

引证文献157

二级引证文献1304

云南植物研究
2005年 第2期

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