大鼠束缚后脑内Fos蛋白的表达

EXPRESSION OF Fos PROTEIN IN RAT BRAINS FOLLOWING RESTRAINT STRESS

目的 探讨大鼠束缚后对大鼠脑内Fos蛋白表达的影响。 方法 将大鼠束缚于小的塑料桶内1、3 或6h,于解束后30min处死,脑组织进行Fos蛋白免疫组织化学染色。 结果 Fos阳性神经元表达于1.前脑:扣 带回、新皮质(尤其是第3和5层)、外侧隔核、杏仁中央核;2.间脑:下丘脑视前区、下丘脑外侧区、视上核、室旁核、 第三脑室室周区、弓状核、丘脑室旁核、外侧膝状体、内侧膝状体;3.脑干:中脑的上丘视性层、中脑导水管周围灰 质、下丘的皮质部;脑桥的臂旁外侧核、蓝斑、A5区;延髓的耳蜗核、延髓内脏带(MVZ)等处。Fos表达的时间规律是 束缚1h最高,3h次之,6h最少。 结论 大鼠被束缚后全脑多处核团的神经元发生不同程度的Fos反应,随着束 缚时间的延长,动物产生适应性,Fos表达减少。

Objective To investigate expression of Fos protein in rat brains following restraint stress. Methods The experimental rats were restrained in a small plastic tub for,1,3 and 6 hours,and were sacrificed at 30 min after removing restraint.Immunohistochemical ABC method was used to observe distribution of Fos protein-like immunoreactive(-LI)products in rats brain.Results Fos-LI neurons appeared in (1)Frontal brain:the cingulum,cortex(especially in third and fifth layer),lateral septal nucleus,central amygdaloid nucleus.(2)Diencephalon:the thalamic paraventricular nucleus,lateral geniculate body and medial genicular body,hypothalamic paraventricular nucleus,supraoptic nucleus,periventricular area of third ventricle,arcuate nucleus.(3)Brain stem:the superficial layer of superior colliculus,periaqueductal gray,cortical area of inferior colliculus,lateral parabrachial nucleus,locus coeruleus,A5 area,cochlear nuclei,medullary viceral zone in medulla oblongata.The expression of Fos-LI neurons peaked in rats restrained for 1h,at 3h,then began to decrease,at 6h,significantly decreased. Conclusion Fos-LI neurons appeared in many areas of brain induced with restraint stress.The number of Fos-LI neurons decreased following restraint time.