室间隔缺损儿童心房肌细胞Cx43蛋白的表达

CONNEXIN 43 EXPRESSION IN ATRIAL MYOCYTES OF VENTRICULAR SEPTAL DEFECT CHILDREN

目的 探讨室间隔缺损儿童心房肌细胞连接蛋白43(Cx43)表达的规律。 方法 应用SP免疫组织 化学和图像分析方法,观测室间隔缺损儿童心房肌细胞Cx43的蛋白表达。 结果 1.正常儿童心房肌细胞Cx43 表达:1～3岁组呈斑点状、带状或链状,主要排列于细胞侧面连接处,闰盘处极少。7～16岁组大量分布于细胞侧面 连接处和闰盘处。2.室间隔缺损儿童的心房肌细胞Cx43表达:1～3岁组除了分布于细胞侧面连接处,还见于细胞 闰盘处及细胞质中,少数无序排列;7～16岁组分布在细胞侧面连接处和细胞质中,闰盘处极少,且无序排列。3.室 间隔缺损儿童心房肌细胞Cx43的蛋白表达量未发生显著变化。 结论 室间隔缺损儿童心房肌细胞Cx43的蛋白 分布形式有改变,表达量无变化。提示,室间隔缺损仅对Cx43的分布形式产生影响。

Objective To investigate expressional pattern of the Connexin 43 in atrial myocytes of ventricular septal defect children. Methods The expressional role of the Connexin 43 in atrial myocytes of ventricular septal defect children were observed and determinated by immunohistochemistry and digital image analysis technology. Results 1.The Connexin 43 granules of 1-3 year-old-group normal children were mainly distributed on cell side surface,which took on beening spot-,belt-or chain-shaped,but seldom at intercalated disks. The Connexin 43 of 7-16 year-old-group normal children were abundantly distributed on cell side surface and intercalated disks. 2.The Connexin 43 of 1-3 year-old-group ventricular septal defect children were not noly distributed on cell side surface but could be detected at intercalated disks and cytoplasm,a few granules were irregular in arrangement.The Connexin 43 of 7-16 year-old-group ventricular septal defect children were distributed on cell side surface and in cytoplasm but seldom existed at intercalated disks,and arranged in irregular order. 3.The experimental results showed insignificant difference in the expressional amount of the Connexin 43 in atrial myocytes between the ventricular septal defect children and normal children. Conclusion The Cx 43 distributional pattern in atrial myocytes of ventricular septal defect children was changed,but its expressional amount was indistinct,which suggested that ventricular septal defect affected only the Cx43 expressional pattern.