CON43蛋白及mRNA在子宫内膜异位症中表达及意义

Expression of connexin43 protein and mRNA in patients with endometriosis and its significance

目的:探讨间隙连接蛋白CON4 3mRNA及蛋白在子宫内膜异位症中的表达及意义。方法:对2 0例子宫内膜异位症内膜用免疫组化法检测CON4 3蛋白的表达,用RT -PCR检测其mRNA表达,并与2 1例其他良性疾病子宫的内膜进行比较。结果:CON4 3蛋白主要在内膜腺上皮细胞浆中表达,内异症组中CON4 3蛋白表达较对照组显著降低;且内异症组中CON4 3mRNA较对照组显著降低。结论:子宫内膜异位症中间隙连接蛋白CON4 3在mRNA水平和蛋白水平较对照组显著降低,可能是导致内膜上皮细胞间的物质和信号传递减低或缺失,内膜细胞在它处游走。

Objective:To study the expression of connexin43 protein,mRNA and its significance in patients with endometriosis.Methods:Endometria were obtained from 20 cases with endometriosis through hysterectomy,and from 21 cases of the control group through hysterectomy and laparoscopic examination and other ways.Immunohistochemical staining with antibody against connexin 43 protein was performed by S-ABC method.But mRNA of connexin 43 was examined by RT-PCR.Results:Connexin43 protein was mainly expressed in the plasma of glandular epithelium of endometrium.There was a significant difference between the study group and the control group.The expression of connexin43 protein and mRNA in endometriosis group was much lower than in the control group.Conclusion:The gap junction induced by connexin43 protein between the glandular epithelium of endometrium is very important for the communication of substances and signals.The loss of gap junction communication caused by the reduction of connexin43 mRNA and protein in endometriosis might be responsible for the movement of endometrial cells to other places.