EAE大鼠脑中ICAM-1、VCAM-1的检测及意义

The detection and significance of ICAM-1 and VCAM-1 in EAE rat brain

目的:实验性变态反应性脑脊髓炎(experimentalallergicencephalomyelitis ,EAE)是研究多发性硬化(multiplesclerosis,MS)发病机制及探索治疗的理想动物模型。本实验通过检测急性EAE模型大鼠脑中细胞间粘附分子- 1(intercellularadhe sionmolecule - 1,ICAM - 1)、血管细胞间粘附分子- 1(vascularcelladhesionmolecule -l,VCAM - 1)的表达以探讨粘附分子在EAE发病过程中的作用及意义。方法:以豚鼠脊髓匀浆加完全福氏佐剂作为抗原,给予Wistar大鼠四足皮内注射,建立EAE模型。应用免疫组织化学技术检测EAE大鼠脑中ICAM - 1、VCAM - 1的表达。与对照组对比作成组t检验来分析其表达是否具有统计学意义。结果:ICAM - 1,VCAM - 1在EAE大鼠脑血管内皮细胞的表达与正常对照组比较有显著性差异(P <0 .0 1)。结论:EAE模型建立方法稳定、可靠。ICAM - 1、VCAM -

Objective:The experimental allergic encephalomyelitis(EAE) is the most suitable animal model for investigation in the pathogenesis and the treatment of EAE.The expressions of ICAM-1,VCAM-1 in Wistar rat brain of acute EAE were investigated to study the mechanism and significance of their expressions in EAE rat brain.Methods:All experimental Wistar rats were inoculated intradermally in feet with emulsion contained guinea pig spinal cord homogenate and complete Freund’s adjuvant.The clinical manifestation and the pathological changes were observed with the aid of light and electron microscope to check the successful establishment of the model.The expressions of ICAM-1 and VCAM-1 were detected by the method of immunohistochemisty.The results of their expressions were compared by T test with controls.Results:Significant difference was found between the experimental group and the controul group in the expressions of ICAM-1 and VCAM-1 ( P <0.01).Conclusion:The method of establishing the EAE model is stable and reliable.ICAM-1 and VCAM-1 may play a key role in the onset of EAE.