下呼吸道感染的阴沟肠杆菌耐药性及基因型研究

Drug resistance and genotyping of Enterobacter cloacae in lower respiratory tract infection

目的:掌握我院下呼吸道医院感染阴沟肠杆菌(EC)耐药谱及分子流行病学特征,指导临床合理用药。方法:对2 8株阴沟肠杆菌进行药敏试验,并采用优化反应体系的随机多态DNA分型(RAPD)技术进行流行病学研究。结果:2 8株阴沟肠杆菌被基因分型为10型,抗生素分型分为18型。结论:基因分型法显示ICU病房有多重耐药菌株EC株爆发流行现象;应加强微生物室的监测工作,为控制预防下呼吸道医院感染、追踪传染源。

Objective:To study the type of drug resistance spectrum and molecular biological characteristics of Enterobacter cloacae which infects lower respiratory tract in hospital.Methods:Drug resistance in 28 strains EC and optimal reaction system of RAPD were used to study their molecular epidemiology.Results:28 strains EC were divided into 10 types by genotype,18 types by resistance.Conclusion:Genotyping demonstrates the outbreak of multidrug resistant strains EC in ICU.This method is accurate,reliable and highly specific.It can not only provide genetic information for controlling,preventing hospital infection and tracing the infection source and the way of dissemination but also direct the clinical doctors to select sensitive antibiotics.