伪狂犬病病毒基因缺失疫苗制苗用毒种特性研究

The Characteristics of Pseudorabies Virus Gene-deleted Vaccine Strain

本试验通过对PRV基因缺失株SA215(gE-/gI-/TK- )细胞培养特性、理化特性及形态发生过程进行研究,来确定gE、gI和TK 基因缺失对病毒特性的影响。结果表明,基因缺失对该毒株在培养细胞的吸附和穿入过程没有影响,但与亲本株相比,表现为生长掩蔽期延长,增殖速度减缓,但能达到相似的增殖滴度,并且基因缺失对病毒的理化特性影响很小。形态发生过程观察结果表明,PRV SA215 株在细胞培养上形态发生正常,能形成感染性病毒粒子,但在由核膜出芽和囊膜形成上受到一定程度的阻碍。本研究为疫苗研制和生产提供指导和依据。

In order to determine the effect of deletion of gE, gI and TK on the characteristics of virion, we studied the property in cell culture, physicochemical characters and the morphogenesis in vero cells of Pseudorabies virus(PRV) gene-deleted vaccine strain SA215. The results indicated that PRV SA215 had the similar process of adsorption and penetration kinetics as its parental strain Fa; however, the course of replication was prolonged. Furthermore, the deletion of gE, gI and TK only has slight effect on physicochemical characters. The comparative analysis of SA215 and Fa on the morphogenesis in cultured cells suggested that the process of budding from nucleus membrane and forming of secondary envelope were delayed or arrested. The results provide guidance for manufacture of PRV gene-deleted vaccine SA215.