坛紫菜别藻蓝蛋白α,β亚基基因的克隆和序列分析

Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis

以野生坛紫菜色素体DNA为模板，通过PCR扩增获得编码坛紫菜别藻蓝蛋白α亚基和β亚基的序列及两者之间的间隔序列，该序列全长为1055bp，其中编码α和β亚基的序列均为486bp，间隔序列为83bp，该序列与GenBank收录的其他4种红藻相关序列的同源性在75．6％～87．4％，与2种蓝藻的同源性分别为66．9％，68．5％，其中编码区同源性更高。

On the basis of the sequences of allophycocyanins from several species in genbank,a pair of degenerate primers were designed and synthesized. A specific fragment about 1 055 bp in size was obtained after PCR amplification using the cpDNA of Porphyra haitanensis as template. Then the purified fragment of DNA was cloned into vector pMD18-T, and sequenced after the identification of the recombinants using PCR and endonuclease digest. The results indicate that α and β subunit genes of allophycocyanin are obtained from Porphyra haitanensis. The fragment size is (1 055 bp), including α and β subunits coding regions (486 bp) each and the spacer between them is 83 bp.The genes were submitted to genbank and their genbank accession number was AY372218.