乙肝病毒感染儿细胞因子血清标志物与HBV DNA的相关性研究

THE CORRELATION SBUCEY BETWEEN CYTOKINES, HBVM AND HBV DNA IN INFECTED HBV CHILDREN

目的 探讨小儿乙肝病毒 (HBV)感染病理过程中细胞因子IL - 10、IL - 12、IFN -γ与HB VM和HBVDNA的关系及意义。方法 95例HBV感染患儿和 30例健康儿童均以双抗夹心ELISA法检测IL - 10、IL - 12和IFN -γ血清水平 ,同时检测HBVDNA和HBVM。结果 HBsAg携带 (ASC)组和慢性乙型肝炎 (CHB)组IL - 10、IL - 12、和IFN -γ水平均显著高于对照组 (P分别 <0 0 5 ,<0 0 1,<0 0 1)。ASC-HBeAg阳性组IL - 10和IFN -γ较HBsAg阴性组明显升高 (P值均 <0 0 5 ) ;CHB -HBeAg阴性组IL -12和IFN -γ较HBsAg阳性组明显升高 (P值均 <0 0 5 )。ASC -高病毒载量组IL - 10和IFN -γ均显著高于对照组 (P值均 <0 0 1) ,CHB -高载量组IL - 10、IL - 12和IFN -γ均显著高于对照组 (P值均 <0 0 1)。ASC组和CHB组IL - 10与IL - 12、IFN -γ均呈显著正相关 (r分别 =0 4 882 ,0 8712 ;0 84 77,0 3816 ) ,IL- 12与IFN -γ呈显著正相关 (r =0 5 376 ,0 5 5 49)。结论 HBV感染患儿存在异常的细胞免疫应答 ,IL -10、IL - 12和IFN -γ均参与了小儿HBV感染的病理生理过程 ,并且与HBVDNA水平密切相关。

Objective To investigate the serum levels of IL-10、IL-12、IFN-γ,HBVM and HBV DNA in children infected HBV.Methods The serum levels of IL-10、IL-12、IFN-γ and HBV DNA and HBVM of95 infected HBV children were measured by speciric-ELISA 30 healthy children were used as the control. Results The serum levels of IL-10、IL-12、IFN-γin ASC groupsand CHB groupswere significantly higher than those in the control(P<0.05,<0.01,<0.01)respectively.The serum levels of IL-10、IL-12、IFN-γ in HBeAg positive ASC groups were higher than those in HBeAg negative ASC groups patients(P<0.05). The serum levels of IL-12、IFN-γ in HBeAg negative CHB groups were higher than those in HBeAg positive CHB group patients(P<0.05). The serum levels of IL-10 and IFN-γ of high virus load ASC group were significantly higher than those in the control(P<0.01).The serum levels of IL-10、IL-12、IFN-γ of high virus load CHB group were significantly higher than those in the control(P<0.01).IL-10 in ASC group and CHB group positively correlated with the serumlevels of IL-12,IFN-γ(r=0.4882,0.8712,0.8477,0.3816 respectively),IL-12 positively correlated with the serum levels of IFN-γ(r=0.5376,0.5549 respectively).Conclusion conclusions There is an abnormal cell-mediated immune response in children infected HBV. IL-10,IL-12 and IFN-γ may play an important role in pathogenesis of children HBV infection and correlate with with the HBV DNA.