睡眠剥夺对人体循环免疫细胞的影响

EFFECTS OF SLEEP BE DEPRIVATED ON PEOPLE CIRCULATING IMMUNE CELLS

目的 研究睡眠剥夺对循环免疫细胞的影响。方法 选择 4 8名志愿者作为实验对象 ,他们均为某医院内科临床医师 ,分别在其连续 3d正常上班后的第 3天 8Am上班时和 2 4h值班整夜未能睡眠时的第 2天 8Am交班时抽取静脉血 ,查血检测血细胞的数量和比例以及循环T细胞亚群和脾淋巴细胞的细胞周期。结果 正常对照组和睡眠剥夺组的白细胞 (7.4 4× 10 12 /L和 5 .0 8× 10 12 /L)、淋巴细胞 (5 .31× 10 12 /L和 4 .18× 10 12 /L)和中性粒细胞 (1.5 4× 10 12 /L和 1.2 1× 10 12 /L)显著减少 (P <0 0 5或P <0 0 1) ;睡眠剥夺组的单核细胞比例 (3.85 %和 6 .5 4% )显著增高 (P <0 0 5 )。睡眠剥夺后的T细胞亚群也有一定程度的减少 ,且差异有显著性。结论 2 4h睡眠剥夺对人体的免疫功能有一定程度的影响。

Objective Objective To study the effects of sleep be deprivated on circulating immune cells.Methods 48 vonlenteer were selected as research objection.Number and proportion of blood cells,T cell subsets and cell cycle of lymphocytes of vonlenteers were determined before and after sleep be deprivated respectly.Results Leukocytes,lymphocytes,and neutrophils in control groups(7.44×10 12/L,5.31×10 12/L,1.54×10 12/L)were significantly higher than that sleep deprivated groups respectly(P<0.05 or P<0.01),and proportion of monocyte (3.85% vs. 6.54%) was increased in sleep deprivation group(P<0.05);There was no significant difference between sleep deprivation and control groups.No changes were found in T cell subsets and cell cycle of lymphocytes in both groups.Conclusion Circulating immune cells,T cell sbsets and lymphocytes are affected by 24h sleep deprivation.