摘要
葡萄汁酵母的菌株YY具有耐高温特性,研究其可能的耐高温机理具有重要的理论和实践意义。其在37℃能正常生长,致死温度为68℃。通过克隆其tps1基因序列,体外扩增后测序,分析蛋白序列的突变。发现在这一菌株的tps1基因序列有13个突变,TPS1蛋白序列存在有三个位点的变异,分别是67位(Gly/Ala)、69位(Glu/Gln)、387位(Val/Leu),这三个变异均增加了海藻糖-6-磷酸合成酶的疏水性,从而增加了酶在高温下的稳定性性。通过这一发现,可以部分解释菌株YY的耐高温机理。
Thermophilic yeast play a key role in the ferment factory.It is very important to investigate the mechanism of yeast enduring high temperature.One thermophilic yeast,named YY,can live under the condition of 37℃.It's death temperature is 68℃.Cloning and analysis of tps1 gene revealed that the protein sequence has three mutation sites.The mutation sites are 67(Gly/Ala),69(Glu/Gln) and 387(Val/Leu).The mutation can enhance the hydrophobic effect of TPS1 ,accordingly,the mutation can also enhance TPS1 stability.Thus,the mechanism of yeast enduring high temperature can be interpreted partly.
出处
《生物技术》
CAS
CSCD
2005年第2期3-5,共3页
Biotechnology
关键词
耐高温
酵母
克隆
序列分析
突变
thermophilic
yeast
cloning
analysis
mutation
