摘要
在纯培养的条件下,对江西德兴铜矿酸性矿坑水中分离出的一株氧化亚铁硫杆菌(Thiobacillus ferrooxidans)的细胞形态、生长条件以及对Fe2+的氧化进行了初步研究。透射电子显微镜检查的结果表明,其成熟菌体大小均一,有较好的运动性;采用光学显微镜对微生物进行菌群观测和利用血小板计数器法对细菌计数的结果表明,在摇床转速为160r/min的条件下,T.f.菌在9K液体培养基中最适生长条件为温度30℃左右,最佳初始pH 2.0;用重铬酸钾滴定法测定铁的结果表明,在摇床转速为160r/min的条件下,pH值1.7,温度30℃时T.f.菌对Fe2+的氧化速率最大,约为0.58g/L·h。
Under pure cultivation conditions,a strain of Thiobacillus ferrooxidans isolated from the acid mine drainage of Jiangxi Dexing Copper Mine was studied with regard to its cellular morphology, growth conditions and effect on the oxidation of Fe^(2+).Examination by transmission electron microscope showed that the mature T.f.bacteria were of uniform size and had quite good ability to move.The observation of the bacteria group by optical microscope and the counting of the bacteria number using the blood platelet counter technique showed that the optimum growth conditions for T.f.bacteria in 9K liquid culture medium at the shaking bath speed of 160 r/min were ca.30℃ with the initial pH 2.0.The determination of Fe by the potassium dichromate titration method indicated that at the shaking bath speed of 160 r/min,and pH 1.7 and temperature of 30℃ the rate of ferrous iron oxidation by T.f.bacteria attained the maximum of about 0.58g/Lh.
出处
《生物技术》
CAS
CSCD
2005年第2期64-66,共3页
Biotechnology
基金
教育部科学技术研究重点项目资助(编号:02052)
关键词
富集
纯培养
氧化亚铁硫杆菌
9K培养基
生长条件
Fe^2+的氧化
concentration
pure culture
Thiobacillus ferrooxidans
9K culture medium
growth conditions
oxidation of Fe^(2+)
