环境生物新技术在土壤微生态研究中的应用

Environmental Biotechnology: Mechanisms and Applications in Soil Micro-ecosystem Investigation

鉴于传统的微生物分离培养技术已不能满足环境微生物研究的需要。为此,在介绍变性梯度凝胶电泳(DGGE)以及荧光原位杂交(FISH)等分子生物学新技术的基础上,对这些技术在土壤微生态研究中存在的问题和发展前景做了展望;提出将这些技术整合应用到复杂土壤的环境微生态研究中,以了解微生物的时空分布、种群结构及其与功能之间的关系。

Due to the traditional microbial techniques can’t meet the requirement for understanding the microbial community in soil micro-ecosystem, it is very urgent to develop new molecular biotechnology to reveal the microbial black box. In this paper, several molecular methods such as fluorescence in situ hybridization (FISH) and denaturing gradual gel electrophoresis (DGGE) etc. were introduced in detail and their application will provide a better understanding of microbial diversity in soil micro-ecosystem and lead ultimately to the improvement of the efficiency of microbial investigation In the end, the existing problems and future development of these molecular methods were also discussed briefly.