新疆维吾尔族妇女宫颈癌中HPV16感染和p53蛋白表达的相关性研究

Correlation between HPV16 Infection and p53 Protein Expression in Cervical Carcinoma in Xinjiang Uigur Women

采用半巢式PCR法检测 80例新疆维吾尔族宫颈癌及 3 4例正常宫颈组织中HPV16DNA ,采用免疫组化法检测p5 3蛋白 ,分析其与宫颈癌发生的相关性 ,探讨HPV16感染和p5 3蛋白表达在新疆维吾尔族妇女宫颈癌发生中的作用。结果显示 :HPV16检出率在宫颈癌中为 80 .0 % ,与对照组 8.8%相比 ,存在显著性差异 ( χ2 =4 9.88,P <0 .0 0 5 ) ;宫颈癌组织中p5 3蛋白表达阳性率为 6 2 .5 % ,也明显高于对照组 ,P <0 .0 0 5 ;p5 3蛋白表达与HPV16感染在统计学上无相关 ( χ2 =0 .0 ,P >0 .0 5 )。表明 :HPV16感染与新疆维吾尔族妇女宫颈癌发生密切相关 ;HPV16感染的宫颈癌组织中p5

To investigate the correlation between HPV16 infection and p53 protein expression in cervical carcinoma among Uigur women in Xinjiang,we detected for HPV16DNA and p53 protein in 80 cases of cervical carcinoma and 34 cases of normal cervical epithelia with semi-nested polymerase chain reaction and immunohistochemistry methods,respectively.Compared with the control gourp (8.8%),the positive rate of HPV16 was 80.0% in cervical carcinoma.In statistical analysis,the difference was significant(χ~2=49.88,p<0.005).The expressions of p53 protein in cervical carcinoma was 62.5%,p<0.05.In cervical carcinoma,there was no correlation between HPV16 infection and p53 protein expression(χ~2=0.0,p>0.05).Our finding showed that HPV16 infection was closely related to cervical carcinoma, and showed that p53 protein expression has increasing tendency in cervical carcinoma with HPV16 infection.