茶叶多糖及其铈配合物对质粒DNA及有机磷农药的降解作用

Effect of Complex Coordinating Tea Polysaccharide with Cerium on Degradation of Plasmid DNA and Organophosphorous Pesticides

以茶叶多糖(TPS)为配体与四价的铈(Ce4+)结合,形成了水溶性的茶叶多糖铈配合物(Ce TPS)。在中性条件下,TPS及Ce TPS对质粒DNA(PlasmidDNA)有一定的裂解作用,对有机磷农药毒死蜱有很强的降解作用。将TPS及Ce TPS应用于大田,可有效降低菠菜中毒死蜱及乐果残留量,比对照低5倍以上,其中Ce TPS处理8d后毒死蜱及乐果残留量均低于国家标准(≤1.0mg·kg-1)。

The results from our previous works proved that complexes coordinating alginic polysaccharides with cerium have remarkable degradation effect for plasmid DNA and the tea polysaccharide (TPS). The recent works show that both TPS and its coordinating cerium (Ce-TPS) can crack plasmid DNA and degrade chlorpyrifos at pH 7.0 and 37 ℃. The results from field experiment show that applying TPS and Ce-TPS on spinach can effectively reduce residual contents of chlorpyrifos and dimethoate in spinach. The residual contents of the chlorpyrifos and dimethoate in spinach applied Ce-TPS are lower than national standard (1.0 mg·kg^(-1)) after 8 d.