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HLA衍生肽RDP1258延长大鼠移植心脏存活时间 被引量：2

Effect of RDP1258 on survival of rat cardiac allograft
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摘要 目的　观察HLA衍生肽RDP1258对大鼠同种移植心脏存活时间的影响。方法人工合成HLA衍生肽RDP1258;建立大鼠心脏腹部移植模型,随机分为4组。(1)对照组:心脏移植前后不用任何药物;(2)环孢素A(CsA)组:心脏移植前后给予CsA灌胃;(3)RDP1258组:心脏移植前后给予PDP1258腹腔注射;(4)RDP1258+CsA组:心脏移植前后联合给予RDP1258和CsA。分别观察人工合成的RDP1258纯度、移植心存活时间及移植心组织的光镜及电镜检查情况。结果RDP1258纯度达95%以上,分子量与理论值相符。大鼠移植心脏存活时间:对照组为(8.00±2.90) d,CsA组为(13.38±3.62)d,RDP1258组为(33.29±10.09)d,RDP1258+CsA组为(85.38±18.34) d。RDP1258组和RDP1258+CsA组与对照组比较,移植心脏存活时间显著延长。RDP1258组和RDP1258+csA组移植心病理改变较轻,超微结构无明显改变。结论RDP1258能抑制急性排斥反应,围手术期给予RDP1258和CsA,能够明显延长大鼠移植心脏存活时间。 Objective To investigate the effects of RDP1258 on survival of rat cardiac allograft. Methods RDP1258 was synthesized and the model of rat heart abdominal transplantation was established. Animals were divided into four groups. Group 1 received no immunosuppression. Group 2 received CsA alone. Group 3 received RDP1258 alone. Group 4 received RDP1258 and subtherapeutic CsA. In all cases RDP1258 was administrated intraperitoneally and CsA was gavaged. Light and electron microscopic examinations were taken . Transplanted hearts were monitored daily by direct palpation. Results The purity of synthesized RDP1258 was over 95 % and the molecular weight was in accord with theoretical value. The histology and the ultrastructure changed little in grafts in group 3 and group 4. Survival of rat cardiac allograft was significantly prolonged in group 4. Conclusions RDP1258 can suppress acute rejection. Perioperative administration of RDP1258 in combination with CsA can significantly prolong survival of rat cardiac allograft.
作者 崔志刚 汪泽厚 岳明 黄赤兵 张艮甫
机构地区 解放军第 解放军空军总医院泌尿外科 第三军医大学新桥医院泌尿外科
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2005年第4期217-219,共3页 Chinese Journal of Organ Transplantation
基金 国家新药基金资助项目(9690105184)
关键词 RDP1258 HLA衍生肽 存活时间 移植心脏 大鼠 心脏腹部移植模型 急性排斥反应 人工合成 移植前 CsA 对照组 腹腔注射 检查情况 显著延长 病理改变 超微结构 围手术期 环孢素 后联合 心组织 分子量 纯度 Peptide synthesis Heart transplantation Immune tolerance
分类号 R654.2 [医药卫生—外科学]
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参考文献15

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同被引文献18

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引证文献2

中华器官移植杂志
2005年 第4期

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