拓扑异构酶Ⅱα在肝细胞癌中表达及意义

Expression of TopoⅡα in Human Hepatocellular Carcinoma and Its Significance

目的探讨TopoⅡα在肝细胞癌中的表达及意义。方法应用免疫组织化学S-P法检测63例肝细胞癌石蜡包埋标本中TopoⅡα的表达情况。结果63例肝细胞肝癌病例均呈现出了TopoⅡα的阳性表达。TopoⅡα表达的阳性率与患者年龄、性别、HBsAg、肝硬化、肿瘤直径、包膜或者分级均无关(P>0.05),但其与血清AFP水平相关(P<0.05)、与血管侵犯有关(P<0.05)。结论TopoⅡα对研究肝癌细胞增殖活性有一定的特异性。

Objective To investigate the expression of TopoⅡα in hepatocellular carcinoma cells and its significance. Methods TopoⅡα expression was detected by immunohistochemistry S-P method in 63 cases of hepatocellular carcinoma. Results All of the 63 hepatocellular carcinoma demonstrated positive nuclear expression of TopoⅡα. The percentage of positive nuclei Topo Ⅱα does not correlate with age, gender, HBsAg, tumor size, fibrous capsule, capsular invasion, or Edmondson's grade(P>0.05). But TopoⅡα expression has a significant association with the AFP level (P<0.05), and hepatic vascular involvement (P<0.05). Conclusion TopoⅡα can be used as a specific marker of hepatic tumor growth.