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ELISA法检测三种类型的类风湿因子

ELISA METHOD OR DETERMINATION OF IgM-, IgG-, IgA-RHEUMATOID FACTOR
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摘要 用ELISA法建立了检测IgM、IgG、IgA三种类风湿因子。在类风湿关节炎(RA)中IgM-RF,IgG-RF,IgA-RF的阳性率分别为67.1%,58.6%,42.8%,明显高于其他结缔组织病,且OD值也高。ELISA法检测IgM-RF的阳性率明显高于乳胶凝集法检测的阳性率,但特异性一样为2.3%,但弥补了乳胶凝集法只能检测IgM-RF的不足,尤其是建立了IgG-RF和IgA-RF的检测,对临床诊断有很大帮助,我们还发现IgA-RF和SS有一定关系。 We set up a ELISA method for the determination of IgM-, IgG-,IgA-type of rheumatoid factor (RF) and report the result in patients with rheumatoid arthritis (RA), systemic lupus erthematosus (SLE), Sjogren syndrome (SS) and other connective tissue diseases.It was found that the positive rates of IgM-RF 67.1%, IgG-RF 58.6%, IgA-RF42.8%, as well as the OD value in patients with RA were higher than that i n patients with other connective tissue diseases.Our results also revealed the specifcity of ELISA for RF was 2.3%,same as the Latex aggutiniation method. Furthermore.there seemed to be a profound correlation of IgA-RF with SS patients.
出处 《张家口医学院学报》 1994年第3期14-15,共2页
关键词 类风湿因子 类风湿性关节炎 ELISA ELISA rheumatoid factor rheumatoid arthritis
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  • 1王加银,冯根宝,武建国.双抗原夹心ELISA法检测类风湿因子[J]临床检验杂志,1986(01).

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