摘要
本文采用ELISA双单克隆抗体夹心法检测14份拟诊为中枢神经系统病毒感染患者脑脊液(CSF)标本中单纯疱疹病毒(HSV)抗原。结果表明:有8份标本出现阳性,即约57％可测出HSV抗原。其中7份标本结果与间接ELISA检测CSF和血清HSV-Ⅰ～Ⅱ型抗体结果比较,两者有较好的一致性。提示ELISA双单克隆抗体夹心法检测临床标本中HSV抗原,具有简便、特异、敏感等优点,4h内即能获得检测结果,适用于HSV性脑炎(HSE)的早期诊断。
Herpes simplex virus(HSV) antigen in CSF was detected by use o f sandwich ELISA oftwo-monoclonal antibodies against HSV,Comparison ofthis methodwith that of indirection ELISA detection of IgG and IgM against HSVin CSF and serum showed that they had better consisteney,but former had many obvious advantages, such as much more rapid sensitive specific and cheeper.
