人乳腺离体肿瘤组织的ATR-FTIR谱鉴别方法的探索

Investigation of Human Breast Tumors Tissues With ATR-FTIR Spectroscopy in Vitro

衰减全反射傅里叶变换红外光谱(ATR-FTIR)用于人乳腺离体肿瘤组织的研究是一种新的应用. 通过对38例人乳腺良、恶性肿瘤的衰减全反射红外光谱的对比研究发现,它们之间存在明显而规律的光谱差 异,反映了乳腺良、恶性病变组织中,蛋白质、核酸和脂类等生物大分子的构型与构象以及相对含量上的差别.研 究表明,ATR-FTIR可以从分子水平上揭示良、恶性肿瘤的分子结构信息,极有潜力成为一种临床原位诊断肿 瘤的新方法.

Attenuated total reflection (ATR)FTIR spectra of breast tumor tissues from 38 female patients have been determined and investigated. In comparison with the benign and normal tumor tissues, the mainly characteristic changes of these malignant tumor tissues in the spectra were observed in the symmetric and asymmetric stretching band of phosphodiester groups, which shifted to short wavenumber about 6 cm-1 in vasPO2-, and 1220cm-1 band appeared in the corresponding second derivative spectra, which intensity increased. And also integral area ratio A1083/A1548 displayed and order of normal < benign < malignant. The absorption band near 1 162cm-1 due to the C-O(H) stretching modes of serine, threonine, tyrosine groups evidently shifted to a higher wavenumber about 6 cm-1, and the collagen absorption peaks at 1 204 cm-1, 1 281 cm-1 and 1 339 cm-1 became lower or disappeared. The symmetric stretching modes of PO32- at 970 cm-1 from the phosphorylated proteins became stronger. The absorbance intensity ratio A1045/A1083 decreased, the A2852/A2873, A2923/A2958 evidently increased. Our finding indicated that in the breast cancer tissues, the degree of hydrogen - bonding of oxygen atoms in the backbone of nucleic increased, the content of nucleic acid increased and that of collagen fibers and glycogen decreased greatly; the proteins became disorder structures with the intermolecular hydrogen-bonding nearly broken; and also there were changes in the packing and the conformational structure of the methylene chains of membrane lipids. These results showed that ATR-FTIR spectroscopy was able to become a powerful tool for the biochemical study and the vivo diagnosis of human breast cancers.