麻黄非麻黄碱部分中黄酮、生物碱和有机酸的分析

Analysis of flavonoids, alkaloids and organic acids in the non-ephedrine alkaloids of the extract of Ma Huang

对麻黄的非麻黄碱部分中的主要化学成分进行了初步研究。以芦丁为对照品、Al(NO3) 3作显色剂 ,采用分光光度法测定了黄酮总量 ;以盐酸麻黄碱为对照品、溴百里香酚蓝作显色剂 ,通过酸性染料比色法测定了生物碱的总量。两种方法均具有快速、灵敏、重现性好的特点。并通过颜色反应鉴定了非麻黄碱部分中有机酸类的存在。研究结果为进一步研究麻黄活性成分。

Two simple, sensitive and reliable methods using UV/Vis spectrophotometry are developed for the determination of the total quantity of flavonoids and alkaloids in the non-ephedrine alkaloids of the extract of Ephedra sinica Stapf (Ma Huang). The existence of organic acids is tested by the characteristic color reaction in the same part. These results provide the experimental basis for the comprehensive development and utilization of Ma Huang and for the change from waste to resource of the non-ephedrine alkaloids of the extract of Ma Huang.