β_1-肾上腺素受体细胞外第二环表达载体的构建与鉴定

Construction and identification of the expression vector for the second extracellular loop of β_1-adrenoceptor

目的构建并鉴定β1-肾上腺素受体细胞外第二环的表达载体。方法应用聚合酶链技术(PCR),从大鼠基因组中扩增出目的基因,与表达载体pGEX-4T-1重组,采用PCR、限制性内切酶酶切及DNA测序进行鉴定。结果本实验通过采用基因克隆技术构建了β1-肾上腺素受体细胞外第二环的表达载体,并对其进行了鉴定。结论本实验为以后表达该功能肽段和研究相应抗体的致病机制奠定了基础。

Objective Construct and identify the expression vector for the second extracellualr loop of β1-adrenoceptor(β1AR).Methods Target gene was got from rat genomic DNA by PCR,then recombined with the expression vector pGEX-4T-1 and its validity was identified using PCR,restricted endonuclease and DNA sequence analysis.Results This experiment revealed that genetic cloning technology can be used for constructing and identifying the expression vector for the second extracellular loop of β1-adrenoceptor.Conclusion This experiment established a basis for producing the required antigen and studying the autoantibody.