谷氨酸在实验诱导皮质发育障碍大鼠脑皮质中的分布

Distribution of glutamate in experimentally induced disorders of cortical development

目的 观察液氮损伤诱导皮质发育障碍大鼠脑皮质中谷氨酸的分布,探讨其与癫痫发生的关系。方法 实验随机分为液氮损伤组、假手术组和正常对照组,建立皮质发育障碍模型,常规HE和Nissl染色,免疫组织化学方法用SABC法。结果 在大鼠脑嘴尾方向形成了一小的脑回,小脑回Glu 阳性着色细胞在损伤组较假手术组表达增加,却无显著差异(q 检验,P>0.05),然而在小脑回周围区Glu阳性细胞表达显著增加(q检验,P<0.05),其他脑皮质区没有明显改变。结论 小脑回周围兴奋性神经递质谷氨酸的增加可能是导致癫痫发生机制之一。

Objective To correlate in situ disorders of cortical development(DCDs) (microgyria) epileptogenicity and the distribution of glutamate.Methods There were three groups in the experiment including freeze-lesioned , sham-operated and normal groups.All rats were allowed to survive for 16-18 weeks before H.E, Nissl Stain and SABC method.Results All freeze-lesioned animals displayed typical cortical malformations consisting of a longitudinal microgyrus. There was change of Glu in cortical dysplasia. Only the optical densities of Glu-immunoreactive neurons located peripheral area of the lesion was significant(q test,P<0.05) between the experimental group and the two control groups.Conclusion Increased expression of Glu surrounding microgyrus could be one of mechanisms in an intrinsic epileptogenicity of cortical dysplasias.