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Fhit蛋白在下咽癌中的表达及其与临床病理的关系

Expression of frigile histidine triad protein in hypopharyngeal carcinoma and its relation with pathology
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摘要 目的:探讨下咽癌组织中脆性组氨酸三联体(fragilehistidinetriad ,FHIT)基因蛋白Fhit的表达以及和临床病理指标的关系。方法:采用兔抗人Fhit蛋白抗体和枸橼酸-微波-SP免疫组化方法检测6 0例福尔马林固定,石蜡包埋的下咽癌标本中Fhit的表达并分析其与组织学分级,淋巴结转移的关系。结果:(1)下咽癌组织Fhit表达阳性率为4 5 .0 % (2 7/ 6 0 ) ,正常下咽组织表达阳性率为88.3% (5 3/ 6 0 ) ,二者差异有显著性(P =0 .0 0 0 )。癌组织Fhit表达强度较正常下咽部组织高13例(2 1.7% ) ;较正常下咽部组织低31例(5 1.7% ) ,其中阴性11例(18.3% ) ,与正常组织相等16例(2 6 .6 % )。Fhit蛋白低表达下咽癌组织中Fhit染色的阳性细胞数量及强度较正常下咽部组织明显减少及降低;(2 )高分化鳞癌Fhit蛋白表达阳性率为6 2 .5 %(15 / 2 4 ) ,中分化鳞癌为36 .8% (7/ 19) ,低分化鳞癌为2 9.4 % (5 / 17) ,各病理分级之间的差异有显著性(rs=- 0 .2 84 3,P =0 .0 2 77) ;(3)无淋巴结转移下咽癌Fhit蛋白表达阳性率为75 % (9/ 12 ) ,伴淋巴结转移癌组为37.5 % (18/ 4 8) ,差异有显著性(P =0 .0 2 )。结论:下咽癌Fhit的表达较正常组织降低,其表达状况和病理分级及淋巴结的转移相关,提示Fhit表达降低可能对下咽癌的演化和进展具有重? Objective: To investigate the expression of fragile histidine triad Fhit gene protein and its possible relationship with clinicopathological indices in hypopharyngeal carcinoma. Methods:Fhit proteins in 60 specimens of formalin-fixed, paraffin-embeded hypopharyngeal squamous cell carcinoma were detected and its relationship to historical grade and lymphatic metastasis were analyzed by rabbit antibody to human Fhit gene protein with citrate-microwave-SP immunohistochemical method. Results:(1)Fhit expression was detected positive in 45%(27/60)cases of carcinoma, and 88.3% (53/60) in normal hypopharyngeal tissue, respectively, there was a significant difference between them (P= 0.000). 51.7%(31/60) of carcinomas showed a marked loss or absence of Fhit expression compared with 26.6%(16/60) matched normal tissue;(2)the expression of Fhit in hypopharyngeal carcinoma was corresponding to histological grade (P=0.0277) ;(3)the carcinoma with expression of Fhit was corresponding to lymphatic metastasis (P= 0.02). Conclusion: The reduced expression of Fhit may be associated with hypopharyngeal squamous cell carcinoma and its historical grade and lymphatic metastasis; it plays an important role in the development and progression of the tumor, and may become a new prognostic marker in hypopharyngeal carcinoma.
出处 《山东大学基础医学院学报》 2005年第2期108-110,共3页 Journal of Preclinical Medicine College of Shandong Medical University
关键词 下咽肿瘤 鳞状细胞 脆性组氨酸三联体基因 免疫组织化学 Hypopharyngeal neoplasms Carcinoma, squamous cell Fragile histidine triad gene Immunohistochemistry
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