骨髓基质细胞移植对大鼠损伤脊髓GDNFmRNA表达的影响

Influence of BMSCs transplantation on expression of GDNF mRNA in spinal cord injury of rats

目的:研究骨髓基质细胞(BMSCs)移植对大鼠损伤脊髓胶质细胞源性神经营养因子(GDNF)表达的影响。方法:将大鼠36只随机分为移植组和对照组,每组3个时相点,每个时相点每组6只动物。移植或手术后24, 72h和7d, 用RT PCR方法对GDNF的表达进行半定量分析。结果:移植组GDNFmRNA24, 72h和7d时都明显高于单纯损伤组(P<0 .05)。结论:BMSCs移植可使损伤脊髓表达GDNF增强。

Objective: To explore the influence of bone marrow stromal cells(BMSCs)transplantation on expression of gliocyte derived neurotrophic factor(GDNF) mRNA in spinal cord injury of rats.Methods:Thirty-six rats were divided into transplantation group and control group.There were 3 time points in each group.The expression of GDNF mRNA were evaluated by using RT-PCR after transplantation or operation. Results:The expression levels of GDNF mRNA in transplantation group were significantly higher than those in control group at the 24-hour,72-hour,and 7-day time points.Conclusion:The transplantation of BMSCs can elevate the expression levels of GDNF mRNA in spinal cord injury of rats.