myr-ψPKC对胃癌细胞MGC803凋亡的调节作用

Relationship between myr-ψPKC and apoptosis induced by vincrestine in human gastric cancer cells MGC803

目的:探讨蛋白激酶C(PKC)抑制剂myr- ψPKC对长春新碱(VCR)诱导胃癌细胞MGC803凋亡的影响。方法:用流式细胞术对VCR及myr -ψPKC作用下的细胞进行周期解析,观察细胞周期的变化。结果:通过细胞的周期解析发现VCR和myr- ψPKC共同作用的细胞其凋亡比例为31 .23%,显著高于VCR单独作用引起的凋亡( 18. 4% ) (P<0 .05)。结论:myr- ψPKC可能通过抑制PKC的活性、抑制胃癌细胞增殖,对VCR诱导的MGC803细胞的凋亡具有协同作用。

Objective: To investigate the effect of protein kinase C (PKC) inhibitor, myr-ψPKC, on apotosis of MGC803 cells induced by vincrestine (VCR).Methods:The cells cycle analysis was determined by flow cytometric assays to study the apoptosis of MGC803 cells exposed to VCR.Results:The ratio(31.23%) of apoptosis cells treated by VCR and myr-ψPKC was significantly higher than that (18.4%) treated by VCR (P<0.05).Conclusion:The myr-ψPKC may cooperate with VCR in apoptosis of MGC803 cells by inhibiting the activity of PKC.