冬凌草甲素对哺乳动物细胞DNA损伤作用的影响

The Effect of Oridonin on DNA Damage in the Mammalian Cells

目的 探讨冬凌草甲素对哺乳动物细胞DNA损伤的抑制作用。方法 用小鼠骨髓嗜多染红细胞(PCE)微核试验和大白鼠肝原代细胞非程序DNA合成(unscheduledDNAsynthesis ,UDS)实验检测冬凌草甲素对哺乳动物细胞DNA损伤的影响。结果 冬凌草甲素可明显地抑制环磷酰胺(CP)诱导的小鼠骨髓PCE微核发生率(P <0 .0 1)及盐酸氧芥(NH2 ·HCl)诱导的小鼠肝原代细胞UDS(P<0 .0 1)。结论 冬凌草甲素具有明显的抑制哺乳动物细胞DNA损伤的作用。

Objective To study the inhibition of Oridonin on DNA damage in the mammalian cells.Methods Micronucleus assay of polychromatic erythrocytes in mice bone marrow and unscheduled DNA synthesis(UDS)in primary cells of liver in rat were used.Results Oridonin could evidently inhibit micronucleus frequencies induced by cydophosphamide (P<0.01) and UDS induced by NH 2·HCl(P<0.01).Conclusion Oridonin has significant inhibition on DNA damage in the mammalian cells.