骨桥蛋白和P21在原发性肝癌中的表达及其临床意义

Expression of osteopontin and P21 in primary hepatic carcinoma and their clinical significance

目的 探讨骨桥蛋白(OPN)和P2 1在原发性肝癌组织中的表达及它们在肝癌转移中的作用。方法 采用免疫组化S P法检测69例原发性肝癌组织中OPN和P2 1的表达情况。结果 ①OPN和P2 1的阳性表达主要定位于癌细胞的细胞浆;OPN、P2 1在69例肝癌组织中阳性表达率分别为72 .5 % (5 0 / 69)和62 .3 % (4 3 / 69)。②在发生转移的肝癌组织中OPN和P2 1阳性表达率分别为84.2 % (3 2 / 3 8)和76.3 % (2 9/ 3 8) ,高于无转移的肝癌组织中OPN(5 8.1% ,18/ 3 1)和P2 1(4 5 .2 % ,14 / 3 1)阳性表达率,差异具有显著性,P <0 .0 5。③两者均阳性表达的3 5例肝癌中有2 4例(74.3 % )已发生转移,与两者均阴性表达的11例肝癌中仅有1例(9.1% )发生转移比较差异有显著性,P <0 .0 5 ,提示OPN和P2 1均阳性表达与肿瘤转移的发生密切相关。④OPN和P2 1的表达与肿瘤的大小、AFP水平、有无HBV感染、肿瘤分化、性别、年龄等因素无关,但与肿瘤侵袭转移的发生有关,P <0 .0 5。⑤OPN和P2 1的表达呈显著正相关(r =0 .2 5 7,P <0 .0 5 )。结论 OPN和P2 1的表达与肝癌侵袭转移的发生有密切关系,两者均阳性表达时对预测肝癌已发生转移具有指导意义。

Objective To investigate the expression of osteopontin (OPN) and P21 in primary hepatic carcinoma(PHC) tissue.Methods Expression of OPN and P21 in primary hepatic carcinoma tissues from 69 patients with primary hepatic carcinoma were detected using SP immunohistochemical technique. Results OPN and P21 expression was mainly located in the cytoplasm of PHC cell. The positive rates of OPN and P21 in primary hepatic carcinoma tissues were 72.5% and 62.3%,respectively.The positive rates of OPN and P21 in primary hepatic carcinoma tissues with metastasis were 84.2%(32/38)and 76.3%(29/38),respectively, which were higher than that in primary hepatic carcinoma tissues without metastasis. OPN and P21 positivity was remarkably correlated with the metastasis in PHC(P<0.05). The positive-expression cases of both OPN and P21 in primary hepatic carcinoma tissued were 35,out of them, 26 cases had metastasis,however,in the negative-expression case of both OPN and P21, only 1 case had metastasis. Both OPN and P21 positive-expression was remarkably related with the metastasis in primary hepatic carcinoma(P<0.05). The positive-expression of OPN and P21 weren't related to the size of tumor, level of α-fetoprotein, HBsAg, gender, age and stage of tumor,while they were correlated with metastasis of tumor.Conclusion Both OPN and P21 are significantly related to the metastasis of PHC and over-expression of both OPN and P21 has some predicting significance of metastasis of primary hepatic carcinoma.