定量检测血清HBV标志物不同阳性组合模式与HBVDNA定量之间的关系

Clinical significance and the relationship between HBV DNA quantity and special models of HBVM through quantity testing

目的 探讨定量检测乙型肝炎病毒血清标志物(HBVM)与HBVDNA定量之间的关系。方法 采用实时荧光定量PCR仪检测HBVDNA ,采用时间分辨荧光免疫分析仪检测HBVM。结果 3 71例乙型肝炎患者表现12种阳性模式,除常见模式外,发现3种HBVM特殊模式,即抗 HBe与HBeAg共存,即抗 HBs与HBeAg或与HBsAg共存模式。含有HBeAg的各种模式病毒含量高,病毒复制活跃,HBVDNA阳性率多数较高。结论 时间分辨荧光免疫分析法是一种敏感、特异方法,HBVM各项指标中,抗原意义远大于抗体意义,只要有HBeAg存在的模式,就代表病毒复制活跃及传染性强。抗 HBe与HBeAg共存或抗 HBs与HBeAg或与HBsAg共存时,抗 HBs不能清除HBV ,抗 HBs及抗 HBe也不代表病毒复制减少。

Objective To explore the clinical significance and the relationship between the HBV DNA quantity and some special models of HBVM through quantity testing.Methods HBV DNA tested with the real-time fluorescent quantitative PCR instrument,and hepatitis B virus markers with time-resolution immunofluorescence.Results 12 models demonstrated in 371 hepatitis B cases and 3 special HBVM models illustrated coexistence of anti-HBe and HBeAg,and that of anti-HBs and HBeAg or HBsAg.In models of HBeAg,more viruses reproduced actively,with large positive proportion in HBV DNA.Conclusion The sensitive,special TRIF testing for HBVM has greater clinical significance.The existenec of HBeAg positive model represents active reproduction.Even with the coexistence of anti-HBe and HBeAg,and that of anti-HBs and HBeAg or HBsAg,anti-HBs can not eliminate HBV,nor can anti-HBs and anti-HBe demonstrate the reduction of virus reproduction.