摘要
目的:提取分离中药赤芍中拮抗内毒素(LPS)的有效成分并进行药理学活性检测。方法:通过生物传感器,结合常规的中药分离技术,分离提取出赤芍拮抗LPS的有效成分,应用鲎实验和ELISA法检测该有效成分对LPS的中和作用。结果:赤芍中的有效成分与LPS具有较高的结合作用,该结合作用具有较强的中和LPS活性,在体外能够显著抑制由LPS介导小鼠RAW 2 6 4.7细胞释放TNF α。结论:以LPS为靶点,应用生物传感器技术,可快速、有效地提取分离赤芍中具有较强的中和LPS活性的有效成分。
AIM: To isolate and extract anti-endotox effective components in Radix Paeoniae Rubra. METHODS: By the biosensor technology, the anti-endotoxin effective components were isolated and extracted with general separate technology of Chinese traditional medicine. Through quantificational mensuration for LPS and inhibition of TNF-α release from endotoxin-stimulated cells in vitro, anti-endotoxin activity of Radix Paeoniae Rubra were elucidated. RESULTS: The effective components had high binding capability with LPS. After incubation with endotoxin and cells, the capability showed anti-endotoxin and inhibition of TNF-α release from endotoxin-stimulated cells in vitro. CONCLUSION: The anti-endotoxin effective components isolated and extracted using LPS as target by the biosensor technology are available in searching anti-endotoxin agents from Radix Paeoniae Rubra. It shows that Radix Paeoniae Rubra has high ability on neutralization with LPS.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第3期326-328,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金资助课题 (№ 3 0 3 71767)
关键词
赤芍
生物传感器
内毒素
提取
分离
Radix Paeoniae Rubra
biosensor technology
endotoxin, extraction
isolation
