蝙蝠葛酚性碱抗氧化和降脂作用的实验研究

Antioxidative and lipid-lowering effects of phenolic alkaloids of Menispermum dauricum

目的:研究蝙蝠葛酚性碱(PAMD)抗氧化和降脂作用。方法:通过TBA法测定大鼠肝匀浆自发和诱导的脂质过氧化产物MDA的量,研究PAMD对脂质过氧化的作用;利用Fenton反应和Marklund反应研究PAMD对羟自由基和超氧阴离子的清除作用;通过建立大鼠高脂血症模型,研究PAMD对高脂模型动物的降脂及抗氧化保护作用。结果:PAMD能抑制自发的和诱导的脂质过氧化产物MDA的生成,对化学体系产生的羟自由基和超氧阴离子有清除作用;能降低高脂模型动物血清及肝中TC、TG、LDL、MDA含量,对血清SOD活性和HDL含量有一定的升高作用。结论:PAMD具有抑制体外脂质过氧化和清除自由基作用,能调节高脂模型动物血脂水平。

AIM: To investigate the antioxidative effects of phenolic alkaloids of Menispermum dauricum (PAMD) and its lowering lipid effects. METHODS:The effects of PAMD on spontaneous and induced liver homogenate lipid peroxidation were studied by TBA method for testing the content of MDA. Reactions of Fenton and Marklund were used to test the scavenging capacity of PAMD on superoxide anion and hydroxyl radicals. The lipid-lowering capacity of PAMD was observed on hyperlipidemic animal model. RESULTS:PAMD inhibited the production of MDA in spontaneous and induced peroxidation of liver homogenate. It also scavenged superoxide anions and hydroxyl radicals. CONCLUSION:PAMD can inhibit the lipid peroxidate in vitro, scavenge free radicals, lower the contents of TC, TG, LDL, and MDA in serum and liver and heighten the activity of SOD and the content of HDL in serum.