粉尘螨脂质体制备和对致敏小鼠免疫功能的影响

Preparation of liposomes encapsulating dermatophagoides farinae antigen and it's influence on immunity of mites sensitized mice

目的:制备粉尘螨脂质体,并探讨其对粉尘螨(抗原)致敏小鼠免疫功能的影响,以了解粉尘螨脂质体脱敏效果及机制。方法:用粉尘螨(抗原)致敏BALB c小鼠,皮下注射不同剂量粉尘螨脂质体,ELISA法分别检测小鼠血清总IgG、总IgE、螨特异性IgG (sIgG)、螨特异性IgE (sIgE)滴度及IL 4、IFN γ水平。结果:致敏组小鼠血清总IgE、sIgE、总IgG、sIgG滴度及IL 4水平较正常组升高(P <0 .0 1) ,IFN γ水平显著下降(P <0 .0 1)。皮下注射粉尘螨脂质体后,小(1μg·g-1体重)、中(3μg·g-1体重)、大(9μg·g-1体重)剂量组小鼠血清IL 4水平较致敏组下降,IFN γ水平显著升高,与正常组相近(P>.0 5 )。总IgE、sIgE均显著下降,并以小剂量组下降最明显,与正常组相近,但中、大剂量组仍未下降至正常水平;小、中剂量组IgG及sIgG滴度与致敏组相比显著升高,尤以小剂量组升高最明显,而大剂量组与致敏组相比无明显差异,但仍较正常组显著升高。结论:所制备的粉尘螨脂质体可以对粉尘螨致敏小鼠产生脱敏作用,其中以小剂量组脱敏效果最好。

AIM: To investigate the changes on immunological function in antigen-sensitized mice injected by dermatophagoides farinaeliposomes (DF) subcutaneously. METHODS: BALB/c mice were sensitized with DF antigen injected subcutaneously. After injected by DF liposomes with 1, 3, and 9 μg·g -1 dosage respectively, the total IgE, total IgG, DF specific IgE(sIgE), DF specific IgG (sIgG), IL-4, and IFN-γ were detected by ELISA. RESULTS: The total IgE, sIgE, total IgG, and sIgG in the serum of sensitized mice increased, the level of IFN-γ reduced and IL-4 increased. After injected subcutaneously by DF liposomes, the total IgE, sIgE and IL-4 in the serum of mice of all the treatment groups reduced, and IFN-γ raised, and the total IgG, sIgG raised in the serum of mice administrated with small and medium dosage, but the low dosage was more obvious. CONCLUSION: DF liposomes have some effects on the immunological function in mite-sensitized mice in the low dosage.