摘要
从保留的室内相对敏感品系分出一个亚品系开始培育朱砂叶螨的甲氰菊酯抗药性品系。连续用药40 代,抗性增长68.5 倍。以此抗性品系(R)和相对敏感品系(S)杂交、回交,研究朱砂叶螨对甲氰菊酯的抗性遗传形式。正交(SR)和反交(RS)F1代的LC-P 线介于S 与R 之间且偏向S 方,显性度DSR和DRS分别为-0.83 和-0.29,表明抗性由不完全隐性基因控制;两D 值(DRS和DSR)95%置信限没有重叠,表明两个D 值(DSR和DRS)存在显著差异,也表明朱砂叶螨对甲氰菊酯的抗性遗传可能存在母体影响或核外效应;对F1 杂合子与亲本的回交(SR♀×S♂和RS♀×R♂)F2代测定表明抗性由单基因控制。
The resistant inheritance mode of Tetranychus cinnabarinus to fenpropathrin were studied based on the resistance selection in laboratory. After 40 generations selection, T. cinnabarinus developed 68.5-fold resistance to fenpropathrin. The methods of cross and back-cross between resistant (R) and sensitive (S) strains were used for exploring the inheritance mode of the resistance of this mite to fenpropathrin. The log (LC)-P equation of F1 got from obverse (SR) and reverse (RS) crosses was intermediate between S and R and inclined to S, the dominant indices DSR and DRS were – 0.83 and – 0.29, respectively, which indicated that the resistance is controlled by the incompletely recessive gene; the 95% confidence limit of this two dominant indices do not superpose showing DSR and DRS have significant difference and the maternal or external karyon effect may be existed in resistance inheritance; the back-cross results of heterozygote F1 with its parents suggested that the separation of progenies F2 (SR♀× S♂ and RS♀× R♂) was consistent with Mendel’s single gene inheritance model.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第4期719-724,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(39970493)
