转基因水稻及其杂交后代对稻纵卷叶螟的田间抗性检测

Resistance of cry1Ac + SCK Transgenic Rice and Its Filial Generation to the Rice Leaf Roller Cnaphalocrocis medinalis

用剥查法,研究了转cry1Ac+SCK 基因水稻及其杂交后代对稻纵卷叶螟(Cnaphalocrocis medinalis)的大田抗性。结果显示,在自然生产条件下,转cry1Ac+SCK 基因的两个株系MSA 与MSB 对稻纵卷叶螟具有极强的抗性。转cry1Ac+SCK 基因稻的杂交后代21S/MSB 与II-32A/MSB 都获得了高抗稻纵卷叶螟性状。MSA、MSB、21S/MSB 与II-32A/MSB 上的稻纵卷叶螟数量、卷叶率、卷叶株率与带虫株率均极显著低于常规对照稻。

Field evaluations were conducted to investigate the resistance of cry1Ac + SCK transgenic rice and its filial generation to the rice leaf roller Cnaphalocrocis medinalis. The results indicated that two transgenic rice lines of MSA and MSB inserted with the combination of cry1Ac and SCK were highly resistant against the rice leaf roller, Cnaphalocrocis medinalis, and the progenies from crosses between cry1Ac and SCK transgenic rice and conventional rice varieties, 21S/MSB and II-32A/MSB showed high resistance to leaf roller C. medinalis. The number, percentage of rolled leaves and leaf-rolled plants as well as plants with C. medinalis of MSA, MSB, 21S/MSB and II-32A/MSB were remarkably lower than those of their conventional control rice varieties, respectively.