大鼠肝卵圆细胞免疫组化定位及超微结构观察

Hepatic oval cells in rat: immunohistochemistry localization and ultrastructure

目的研究大鼠肝组织卵圆细胞的定位及其超微结构。方法建立SD大鼠卵圆细胞增生模型,用胆管上皮分化标志CK18、19和干细胞标志CD34作组织免疫组化染色,采用透射电镜观察超微结构。结果组织免疫组化发现大鼠卵圆细胞主要分布于汇管区,部分分散于肝小叶内,根据组织和细胞透射电镜超微结构特点,发现卵圆细胞有三型,Ⅰ型细胞体积较小、7μm左右,核大、胞质少,细胞器少,此为较为原始的卵圆细胞。Ⅱ型细胞体积稍大,8μm左右,胞质稍多,有部分细胞器。Ⅲ型细胞体积更大,9μm左右,细胞器较多。结论大鼠肝卵圆细胞位于汇管区,超微结构观察发现肝卵圆细胞可能是肝脏干细胞。

Objective To investigate the location and ultrastructure of hepatic stem cells in adult rat. Methods Proliferation of hepatic oval cell of the rat was accomplished. Identification was made with immunohistochemistry with CK18, 19 and CD34 as markers. Ultrastructure of hepatic stem cells was observed with transmission electron microscope. Results The majority of hepatic oval cells were localized in the Hering channel, but some of them were distributed in the hepatic lobules. Electron microscopy revealed three types of oval cells. TypeⅠ cell was small, 7μm in size, with large nucleus but small amounts of cytoplasm and organelles. The cell was recognized as primitive oval cell. Type Ⅱ cell was larger, measuring 8μm in diameter, containing more cytoplasm and organelles. Type Ⅲ cells were larger than both of above cells, and they contained more organelles. Conclusion Hepatic oval cells in adult rat are localized in Hering channel, and they may be stem cells hepatocytes.