瘦露螽配子发生中一氧化氮合酶的分布

Distribution of the nitric oxide synthase during gametogenesis in Phaneroptera gracilis Burmeister

利用还原型烟酰胺腺嘌呤二核苷酸 (NADPH) 黄递酶组织化学方法, 对瘦露螽 Phaneroptera gracilis Burmeister配子发生中一氧化氮合酶 (nitric oxide synthase, NOS) 分布进行了定位研究。结果表明, 一氧化氮合酶阳性反应发生在瘦露螽精子发生中的各级生精细胞的胞质中, 成熟精子呈阴性。各级未成熟卵母细胞胞质均呈一氧化氮合酶阳性反应, 胞质着色为深蓝黑色, 核区不明显。随着卵黄颗粒的逐渐形成, 胞质中的一氧化氮合酶阳性产物逐渐减少, 直到卵黄颗粒完全形成。卵泡细胞在卵黄颗粒形成之前呈一氧化氮合酶阴性反应, 在卵黄颗粒完成后, 卵泡细胞的胞质中开始呈一氧化氮合酶阳性反应, 直至卵壳的形成。提示一氧化氮参与了瘦露螽配子发生。

The distribution of nitric oxide synthase (NOS) during gametogenesis in Phaneroptera gracilis Burmeister was studied with NADPH-diaphorase histochemical method. The results showed that nitric oxide synthase positive reaction could be found in the cytoplasm of permatogenic cells in the different stages of the gametogenesis in P. gracilis, while NOS negative reaction appeared in matured sperms. There were NOS positive reactions in the cytoplasm of immatured oocytes in all stages. Usually, cytoplasms were stained with some heavy black while nuclear regions were not obvious. With the formation of yolk granules, the products of NOS positive reactions diminished gradually in cytoplasm until yolk granules formed completely. The follicle cells showed NOS negatively before the formation of yolk granules. After the yolk granules formed completely, the cytoplasm of follicle cells started showing NOS positively until the formation of chorion.