超声微泡促进肿瘤细胞报告基因转染

Enhanced gene transfection in tumor cells by ultrasound and microbubbles

目的 探讨超声破坏微泡造影剂后对β-半乳糖苷酶报告基因在人肝癌细胞(QGY)中转染的影响。方法 将培养QGY细胞转于2 4孔培养板后,分为5组,分别为单纯加入质粒组(D) ;质粒+微泡组(D +M) ;质粒+超声组(D +U) ;质粒+超声+微泡组(D +U +M) ;脂质体+质粒组(L +D) ;进行β-半乳糖苷酶质粒DNA的转染。2d后,进行β-半乳糖苷酶染色,测定各组细胞转染率。结果 单纯质粒组细胞转染率为4.92 % ;质粒+微泡组转染率为6 742 % ;质粒+超声组转染率为2 9.73 % ;质粒+超声+微泡组转染率为5 0 .88% ;脂质体+质粒组转染率为12 .15 % ;结论 超声破坏微泡造影剂可促进报告基因β-半乳糖苷酶质粒在QGY细胞的转染,为肿瘤基因治疗提供一种新型基因转移系统。

Objective To investigate the feasibility of using ultrasound- mediated microbubbles destruction for tumor gene transfection.Methods QGY cells were divided into 5 groups after cultured in a 24 well plate: plasmid DNA group(D); plasmid DNA+microbubbles group(D+M); plasmid DNA+ultrasound group(D+U); plasmid DNA+ultrasound+microbubbles group(D+U+M); liposome+plasmid DNA group (L+D), β-galactosidase plasmid DNA was transfected into the cells with or without ultrasound, microbubble, and liposome. 48 hours later, β-galactosidase staining was applied to observe the expression of plasmid.Results In the group of D, the positive rate of blue cells was 4.923%; in the group of D+M, the rate of blue cells was 6.742%; in the group of D+U, the rate of blue cells was 29.73%; in the group of D+U+M, the rate of blue cells was 50.88%; in the group of L+D, the rate of blue cells was 12.15%.Conclusion Ultrasound-mediated microbubbles destruction could enhance the transfection of report gene in QGY cells, this provides a new safe and effective gene delivery system for tumor gene therapy.