CCAAT/增强子结合蛋白在皮脂腺发育中的表达及意义

Roles of CCAAT/enhancer binding protein and genes in human sebaceous gland differentiation

目的 检测皮脂腺中C/EBPα和C/EBPβ的表达,探讨其与皮脂腺分化的关系。方法 利用RT PCR和免疫组化染色,分别检测C/EBPα和C/EBPβmRNA及蛋白在胚胎和成人皮脂腺中的表达情况。结果 C/EBPαmRNA的表达在胚胎期较弱,随着分化的进行逐渐升高。C/EBPβmRNA的表达则维持在同一水平。免疫组化检测到C/EBPα和C/EBPβ在胚胎期皮脂腺细胞核内均有表达,在成人皮脂腺则表达在基底层细胞的胞核内。结论 C/EBPα和C/EBPβ的蛋白表达与皮脂腺分化密切的关系。

Objective To observe the expressions of C/EBPs mRNA and protein in the sebaceous gland and to study the relationship between C/EBPs and the differentiation of sebocytes. Methods RT-PCR and immunhischemistry were used to detect the expressions of C/EBPs mRNA and protein in the embryo and adult sebaceous glands. Results The lowest expression of C/EBPα mRNA in the sebaceous gland was found in embryonic period, but increased gradually during the developmental stages. The expression of C/EBPβ mRNA in the sebaceous gland showed different expression patterns, i.e. it maintained at low level in all of the developmental stages. Expressions of C/EBPα and β protein were found in the nuclei of sebocytes in embryonic period but in the basal cell layer of sebaceous glands in maturation phase. Conclusion The expression patterns of C/EBPs are different in the sebaceous gland from embryonic to adult stages, suggesting that C/EBPα and β may play important roles in the development of human sebaceous gland.