天疱疮患者头发毛囊与皮损中自身抗体沉积的比较

Comparison of the Antibodies Deposition in Plucked Hair Follicle and Lesion of Pemphigus Patients

目的通过检测天疱疮患者拔出的头发上残留毛囊和皮损中天疱疮抗体的沉积情况,建立一种简便、迅速的检测方法。方法直接免疫荧光方法测定21例天疱疮患者拔出的头发上残留毛囊中和皮损处天疱疮抗体的沉积。结果FITC标记的IgG抗体在19例天疱疮患者毛囊外毛根鞘的1～2层呈明显连续的网状或珠状沉积,2例稳定期天疱疮患者为阴性。在检测的19例天疱疮患者中17例皮损表皮内棘细胞间IgG呈网状沉积,2例稳定期患者为阴性。在8例大疱性类天疱疮患者及10例正常人无网状沉积。结论检查毛囊处天疱疮抗体沉积的实验方法简便、快速,无须切片,与皮损检查结果一致,有可能成为天疱疮诊断的一种常规检测方法。

Objective To develop a simple and fast method for the immunopathological diagnosis of pemphigus using placked hair of the patients.Methods The pemphigus antibody deposition in the follicles of depilated hair samples from 21 cases of pemphisus patients, 8 cases of bullous pemphigoid patients and 10 healthy controls were examined by direct immunofluorescence.Results Reticular fluorescence in the 1-2 layers of keratinocytes could be seen in the outer root sheath of pemphigus patients, but not in bullous pemphigoid patients or healthy controls.Conclusion Immunofluorescence of the depilated hair may be a simple and fast method in the diagnosis of pamphigus. It can be examined frequently.