中药尿路清合剂体外抗沙眼衣原体活性研究

Inhibitory Activity of Niaoluqing for Urogenital Chlamydia Trachomatis in Vitro

目的了解中药尿路清合剂体外抗泌尿生殖道沙眼衣原体的活性。方法尿路清合剂浓度为1g/ml,10株沙眼衣原体为来自性病门诊的临床株,未进行血清学分型,应用微量McCoy细胞培养法,检测中药尿路清体外抗沙眼衣原体的活性。结果尿路清有抗泌尿生殖道沙眼衣原体的活性,当浓度为50～200mg/ml时能显著减少包涵体数目,其最大抑制率达94%,随着中药浓度的升高,衣原体包涵体的体积逐渐减小、数量逐渐减少,最后消失。结论尿路清有体外抗泌尿生殖道沙眼衣原体的作用。

Objective To detect the sensitivity of urogenital chlamydia trachomatis (CT) to Niaoluqing,a kind of traditional Chinese medicine oral liquid.Methods The concentration of Niaoluqing liquid is 1g/ml, 10 CT clinical strains are from STD clinic ,without determining their serological type.The inhibitory activity of Niaoluqing to CT was detected by microculture technique of Mccoy cell in vitro.Results Niaoluqing had inhibitory activity for urogenital CT, having reduce the number of inclusion with 50-200mg/ml concentrations notably,the number and volume of CT.inclusions reduced gradually and disappeared finally with the arising of concentration.Niaoluqing liquid have been found no cytotoxicity to Mccoy cells and no apparently effect on the growth of these cells.Conclusion Traditional Chinese medicines possess some inhibitory activity for urogenital CT.