五氯酚对大鼠睾丸支持细胞影响的研究

Effects of Pentachlorophenol on Rat Sertoli Cells

目的:检测五氯酚(PCP)对体外培养的大鼠睾丸支持细胞的毒性作用。方法:35日龄雄性SD大鼠4只,采用MTT法检测浓度为10-2、10-1、1、10μmol/LPCP对大鼠睾丸支持细胞活力的影响以及AnnexinV/PI流式细胞术检测PCP对大鼠支持细胞的毒性作用。结果:支持细胞的活力随PCP浓度的提高而明显降低并呈时间依赖效应;随浓度增高,细胞由胞质丰富、胞核饱满变为胞质稀疏、胞核皱缩、细胞数目减少,进而细胞界限不明显乃至消失;流式细胞仪检测结果表明,PCP导致坏死的支持细胞比例增加。结论:PCP可引起大鼠睾丸支持细胞坏死,细胞毒性作用明显。

Objective: To detect the toxic effects of pentachlorophenol (PCP) on cultured rat Sertoli cells. Methods: The viability of Sertoli cells was detected and morphological examination was performed, followed by flow cytometric assay to evaluate the toxic effect of PCP on rat Sertoli cells. Results: MTT assay showed that PCP induced a concentration- and time-dependent decrease in Sertoli cell viability. Flow cytometric assay revealed that the number of dead Sertoli cells grew along with increased exposure to PCP. Conclusion: PCP, with obvious cytotoxic effects, can cause necrosis of Sertoli cells in vitro. Natl J Androl,2005,11(4):261-263,268