Attractin蛋白和mRNA在不同日龄大鼠睾丸中的表达

Distribution of Attractin Protein and mRNA in the Testis of Rat of Different Ages

目的:已知成熟大鼠睾丸组织中有Attractin蛋白的广泛表达,本文旨在进一步探讨Attractin蛋白和AttractinmRNA在各年龄段大鼠睾丸组织中的分布。方法:取出生第1d(新生鼠)、第5d(青春前期)、第20d(青春中期)、第50d(青春后期)及第70d(成年期)大鼠睾丸和附睾组织固定,采用酶免疫组织化学和原位杂交方法检测Attractin和AttractinmRNA在大鼠睾丸组织中的表达。结果:免疫组化显示各年龄段大鼠睾丸生精小管和间质细胞、管周肌样细胞上均有Attractin蛋白的表达,分布于胞膜和胞质,精子和附睾上未见表达。随着日龄的增加,间质细胞的表达逐渐增强。原位杂交显示各年龄段大鼠睾丸生精小管和间质细胞、管周肌样细胞、支持细胞上也均有AttractinmRNA阳性棕色颗粒杂交信号,主要表达于胞核及胞质。且随着日龄的增加,睾丸生精细胞表达略强于间质细胞。精子和附睾上未见表达。结论:AttractinmRNA和Attractin蛋白在各日龄大鼠睾丸组织中均有广泛的分布,且分布一致。提示各日龄大鼠睾丸组织都具有合成Attractin蛋白的能力。其生理功能和机制尚有待进一步探讨。

Objective: Given the fact that the Attractin protein expresss widely in the testis of mature male rat, this paper aims at studing the distribution of the Attractin protein and Attractin mRNA in the testicular tissue of rats of different ages. Methods: Testes and epididymides were obtained from newborn(8 hours after birth), prepubertal(5 days), pubertal(20 days), postpubertal(50 days) and mature(70 days) Wistar rats; the tissues were fixed and the Attractin protein and mRNA detected respectively by immunohistochemical and in situ hybridization techniques. Results: The Attractin protein and mRNA expressed respectively within Leydiy cells, primitive spermatogonia, primary spermatocytes, spermatids, Sertoli cells and peritubular myoid cells. The Attractin protein stain was mainly located on the cell membrane and cytoplasm, and Attractin mRNA distributed in the nucleus and cytoplasm. There was no immunopositive staining in spermatozoa and epididymides. Conclusion: The Attractin protein and Attractin mRNA are both expressed widely in the testis of rats of different ages. It suggests that the rat testis has the ability of synthesizing the Attractin protein throughout sexual development, but its physiological function and biochemical mechanism yet remain to be further studied. Natl J Androl,2005,11(4):264-268