肌源细胞自体移植治疗前列腺术后尿失禁可行性的实验研究

Feasibility of Muscle-Derived Cell Autotransplantation as a treatment for Post-prostatectomy Urinary Incontinence

目的:探讨自体骨骼肌肌源细胞(MDC)自体移植在前列腺术后尿失禁治疗中的可行性。方法:采用差速贴壁法分离、纯化培养6只雌性SD大鼠的MDC,然后用携带LacZ基因的腺病毒载体转染MDC,待转染成功后将约5×106携带LacZ基因的MDC自体注射于膀胱颈周围,注射后5、15d处死大鼠,取出膀胱及后尿道,注射部位组织行组织学检查及Xgal染色。结果:在注射后5、15d时注射部位无明显炎症改变及炎性细胞浸润,局部细胞层数增多。经Xgal染色显示各时间点均可见大量的细胞被蓝染,提示移植细胞在体内成活。结论:MDC自体膀胱尿道周围注射可长期存活,提示自体MDC移植有可能成为前列腺术后尿失禁治疗的一种有效方法。

Objective: To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence. Methods: Skeletal muscle-derived cells(MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5×10^(6) of thetransduced cells were injjected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining. Results: At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells. Conclusion: Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence. Natl J Androl,2005,11(4):272-274,277