复方鳖甲软肝片对输尿管结扎大鼠肾组织TGF-β_1蛋白及其mRNA表达的影响

Effects of Fufangbiejiarangan Tablets on expression of TGF-β_1 and mRNA in rat renal cortex after unilateral ureteral obstraction

目的探讨复方鳖甲软肝片对大鼠单侧输尿管结扎(unilateralureteralobstruction,UUO)肾组织转化生长因子β1(TGFβ1)蛋白及其mRNA表达的影响。方法建立UUO肾间质纤维化模型,随机分为正常对照组、假手术组、模型组、苯那普利组和复方鳖甲软肝片低、中、高剂量等7组。手术后4周处死大鼠,无菌取肾,制作冰冻切片,采用免疫组织化学和原位杂交方法,分别检测肾组织TGFβ1蛋白及其mRNA表达,使用图像分析系统对结果进行处理,采用SPSS11.5统计软件统计分析。结果模型组肾小管上皮细胞和肾间质其他细胞浆内,有少量的TGFβ1蛋白及其mRNA棕黄色颗粒状阳性反应物;苯那普利对照组细胞浆中棕黄色颗粒状阳性反应物明显减少,与模型组比,P<0.01;复方鳖甲软肝片治疗组细胞浆中棕黄色颗粒状阳性反应物明显减少,不同剂量组呈剂量依赖关系,与模型组比,P<0.01。结论TGFβ1是导致肾间质纤维化的重要因素,复方鳖甲软肝片可以通过下调TGFβ1蛋白及其mRNA的表达,对UUO肾间质纤维化大鼠肾脏起一定的保护作用。

Objective To study the effects of Fufangbiejiarangan Tablets (FT) on the expression of transforming growth factor-β_1 (TGF-β_1) and mRNA in rat renal cortex. Methods To establish the rat models of renal interstitial fibrosis after unilateral ureteral obstraction(UUO). The rats were divided randomly into 7 groups: the normal group, the pseroperative group, the model group, the Benazepril group, the treatment group 1 with high dose of FT, the treatment group 2 with middle dose of FT and the treatment group 3 with low dose of FT. After the operation for four weeks the expression of TGF-β_1 and mRNA in rat renal cortex were detected respectively by immunohistochemistry and in situ hybridization. The graphic analysis system and SPSS 11.5 statistics software were used to treat the results. Results In the model group the level of TGF-β_1 and the yellow granular positive reactant of mRNA was low in the renal tubular epithellal cell and renal interstitial cell. In the Benazepril group the level of yellow granular positive reactant were obviously decreased compared with the model group (P<0.01). In the treatment groups the level of yellow granular positive reactant were obviously decreased compared with the model group (P<0.01). The treatment group 1, 2 and 3 had different dose dependency respectively. Conclusion TGF-β_1 may play an important role in renal interstitial fibrosis. FT has a protective effect on rat kidney with renal interstitial fibrosis through decreasing the levels of TGF-β_1 and mRNA.