摘要
目的检测清开灵有效组分在体外模拟血脑屏障中的透过率。方法应用脑微血管内皮细胞单层培养模型体外模拟血脑屏障,用高效液相色谱-质谱(LC/MS)分析方法,检测清开灵有效组分栀子苷、黄芩苷、牛胆酸、猪去氧胆酸、去氧胆酸在24h内的透过率。结果透过率从高到低依次为栀子苷、牛胆酸、猪去氧胆酸和去氧胆酸。在滤液中未检测到黄芩苷。结论清开灵有效组分在体外模拟血脑屏障中有较高的透过率,其透过机制和药理作用值得进一步研究。
Objective To determine the permeation rate of effective components of Qingkailing (ECQ) through an in vitro blood-brain barrier (BBB) model. Method To establish an analog in vitro BBB by using mouse model of microvascular endothelial cells monostratal culture. The permeation rate of ECQ (including geniposide, baicalin, cholic acid, hyodeoxycholic acid and dexocholic acid) in 24 hours is detected by means of LC/MS analysis. Result The order of permeation rate is geniposide, cholic acid, hyodeoxycholic acid and dexocholic acid. Baicalin is not detected in the colature. Conclusion The effective components of Qingkailing have a higher permeation rate in an analog in vitro BBB. The mechanism and pharmacological effect are worth studying further.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2005年第2期26-28,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家重点基础研究发展规划项目(No.G1999055404)
关键词
清开灵
有效组分
血脑屏障
透过率
Qingkailing
effective components
blood-brain barrier
permeation rate
