瓜蒌薤白汤对肺纤维化大鼠肺组织中转化生长因子β_1表达的影响

Effect of Gualouxiebai Decoction on TGF-β_1 expression of lung in rats with pulmonary fibrosis

目的探讨瓜蒌薤白汤防治肺纤维化作用及机理。方法用平阳霉素复制大鼠肺纤维化模型后,用药组每天灌胃瓜蒌薤白汤,28d后处死,观察、比较各组大鼠肺部病理组织学改变及肺组织中转化生长因子(TGFβ1)表达。结果模型组大鼠肺泡炎及纤维化程度、肺组织中TGFβ1表达均明显高于正常对照组及用药组(P<0.05)。结论瓜蒌薤白汤能明显减轻平阳霉素所致的大鼠肺泡炎及纤维化程度,抑制肺组织中TGFβ1过度表达。

Objective To investigate the effect and mechanism of Gualouxiebai Decoction for preventing and treating the pulmonary fibrosis. Method The rat models of pulmonary fibrosis was established by bleomycinA5 and then divided into 3 groups. The treatment group was given Gualouxiebai Decoction daily for 28 days. The histopathological changes and the expression of transforming growth factor-β_1 (TGF-β_1) in rat lung were observed and compared.Result The degree of the alveolitis and pulmonary fibrosis in the model group was more serious than that of the normal control group and the treatment group (P<0.05). The level of TGF-β_1 in rat lung tissue in the model group was also significantly higher than that of the normal control group and the treatment group (P<0.05). Conclusion Gualouxiebai Decoction can significantly alleviate the degree of alveolitis and pulmonary fibrosis in rats and inhibit the excessive expression of TGF-β_1.