红花黄色素缓解心肌缺血大鼠心功能下降作用的研究

Remittent Effect of Safflor yellow on rat cardiac insufficiency induced by myocardial ischemia

目的观察大孔树脂层析法制得的红花黄色素(SafflorYellow)缓解心肌缺血大鼠心功能下降的作用。方法大孔树脂层析法制备纯度较高的红花黄色素,大鼠每天腹腔注射此药后腹腔注射异丙肾上腺素(Isoprenaline,ISO)致心肌缺血,第3天经右颈动脉插导管,记录给药及造模前后心室内压变化数据并进行分析;取大鼠心尖组织切片PTH染色观察。结果红花黄色素反复给药可明显缓解ISO多次注射致心肌缺血大鼠左心室舒张末期压的上升(P<0.05),且该药可缓解上述心肌缺血大鼠心室内压最大变化率(+dp/dtmax及-dp/dtmax)、心室峰压、发展压、心肌收缩成分缩短速度、心室峰压×心率等指标下降趋势;光镜观察结果表明,红花黄色素对上述缺血心肌的组织变性未见明显抑制作用。结论红花黄色素具缓解ISO所致心肌缺血大鼠心功能下降的作用。

Objective To observe the effect of safflor yellow made with the chromatography of macroporous resin on rat cardiac insufficiency induced by myocardial ischemia (MI). Method The rats were given daily safflor yellow injection then injected isoprenaline (ISO) to establish the MI model. After three days a catheter was inserted through the right carotid of model. The ventricular pressures before and after the model establishment and the injection were recorded and analysed. The tissue sections of rat apex were stained with PTH and observed with the microscope. Result The increase of left ventricle end-diastolic pressure of MI rat induced by repeated ISO injection could be remitted obviously by safflor yellow (P<0.05). safflor yellow could also control the decrease of some indexes, such as +dp/dtmax, -dp/dtmax, VPP, DP, Vpm and VPP×HR. The inhibition of safflor yellow on the myocardial metamorphosis had not been observed. Conclusion safflor yellow is effective to inhibit the cardiac insufficiency with MI induced by ISO injection.