川芎药材有效成分鉴别及其含量标准研究

Determination of active principle and content standard for rhizoma of chuanxiong

目的对川芎药材有效成分进行鉴别及其含量标准研究。方法TLC法鉴定川芎药材中的藁本内酯,并用HPLC法对药材中的藁本内酯和阿魏酸进行含量测定。结果川芎药材中均能鉴定出藁本内酯,药材中藁本内酯和阿魏酸的含量分别不低于1.50%和0.05%。结论TLC鉴别方法专属性强;含量测定方法精密度高,重现性好,可用于川芎的质量控制。

Objective To determine the active principle and study the content standard for rhizoma of chuanxiong(RC). Method TLC was used to identify the content of ligustilide and HPLC was used to assay the contents of ligustilide and ferulic acid in RC. Result Ligustilide could be identified in RC.The contents of ligustilide and ferulic acid should not be lower than 1.50% and 0.05% respectively. Conclusion TLC is a specialized method and HPLC has a higher accurate and better repeated character for the assay.They can be used in the qualitative_quantitative control of RC.