益气活血剂对兔胸主动脉粥样硬化形成的作用

Effects of prescription for supplementing qi and activating blood circulation on the antiatherogenesis in rabbit aorta

目的探讨中药益气活血剂对高脂饮食兔动脉粥样硬化的预防及其作用机制。方法取纯种新西兰大白兔50只,随机分为4组:正常对照组、高脂模型组、益气活血预防组及益气活血治疗组,观察时间为2月。治疗前后检测血脂,治疗结束后分别取主动脉组织作脂质定量,测胸主动脉粥样硬化斑块面积和内膜、血管壁厚度等。结果①益气活血剂治疗可以降低高脂模型组血胆固醇、甘油三酯、低密度脂蛋白胆固醇,其中甘油三酯水平显著降低(P<0.01)。②益气活血剂治疗可减少高脂模型组动脉粥样硬化的形成,使内膜面积、厚度减少(P<0.05),但对管腔面积无明显影响(P>0.05)。③镜下观察发现,高脂模型组主动脉弓内膜充满泡沫细胞,壁上可见明显的粥样斑块;益气活血治疗组动脉壁脂质沉积相对较轻。结论益气活血剂具有降低血脂和抑制动脉粥样硬化形成的作用。

Objective To determine whether prescription for supplementing qi and activating blood circulation could prevent rabbit fed with high-fat diet from atherosclerosis and the mechanism. Methods Fifty New Zealand rabbits were randomly divided into 4 groups:normal control group, hyperlipidemia control group, traditional medicine prevention group and traditional medicine treatment group. Each group was observed for two months. After treatment, aortic tissue was taken for quantitative estimation of lipid, and serum lipid was detected before and after treatment. Thoracic aorta was prepared for measurement of the aortic plaque area, wall and intima thickness. Results (1)Prescription for supplementing qi and activating blood circulation played an important role in lowering blood TC, TG and LDL-C in hyperlipidemia control group, especially in lowering TG(P<0.01). (2)This prescription could inhibit the atherosclerosis formation in hyperlipidemia control group, thus decreased the area and thickness of the intima(P>0.05), but it had no effect on the area of lumen (P<0.05). (3)The aortic intima was full of foam cells in hyperlipidemia control group underscope and plaques were obviously seen, while the extent of lipid aggradation in the arterial wall in treatment group was relatively less severe. Conclusions Prescription for supplementing qi and activating blood circulation can lower serum lipid and inhibit atherosclerosis formation.